Synthesis and Characterisation of Dual Functional Nanoparticles for Probing the Role of Reactive Oxygen Species in Cellular Function

Lead Research Organisation: University of Hull
Department Name: Physical Sciences

Abstract

The proposal concerns the synthesis and characterisation of dual functional nanoparticles for studying the response of cells to Reactive Oxygen Species (ROS). ROS have been implicated in ageing, heart disease, and recently, in the onset of Alzheimer's disease. The nanoparticles will contain a dye which responds to changes in calcium ions. Calcium ions are an important species which the cell uses to send messages from one site to another. Our dual functional nanoparticles will have the ability to sense changes in calcium ions in their immediate environment within the cell, but what differentiates them from other similar species is the ability to generate bursts of ROS when excited with light. The ROS generating component will be attached to the nanoparticles after they are formed and are composed of molecules called porphyrins. The ability to generate ROS by porphyrins is currently exploited in the Photodynamic Therapy of diseases such as cancer and age related macular degeneration. By changing the chemical structure of the porphyrins and then attaching them to the nanoparaticle we also plan to cause them to accumulate at important structures within the cells, such as the mitochondria. Currently, no similar tools are available for studying ROS in cells and it is anticipated that the successful development of our system will generate important new data relating to how cells respond to ROS.

Publications


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Giuntini F (2014) Conjugatable water-soluble Pt(II) and Pd(II) porphyrin complexes: novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering. in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Giuntini F (2011) Synthetic approaches for the conjugation of porphyrins and related macrocycles to peptides and proteins. in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
 
Description Development of novel probes for oxidative stress
Sector Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal
 
Description Dr A. MacRobert 
Organisation University College London (UCL)
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Collaboration with University College London
Start Year 2012
 
Description Prof. Beate Roder 
Organisation Humboldt University of Berlin
Country Germany, Federal Republic of 
Sector Academic/University 
PI Contribution Collaboration
Start Year 2012
 
Description Prof. Nonell Santi 
Organisation University of Barcelona (UB)
Country Spain, Kingdom of 
Sector Academic/University 
PI Contribution Collaboration with University of Barcelona
Start Year 2012
 
Description Health Research Board Ireland 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Primary Audience Participants in your research or patient groups
Results and Impact Panel Membership . Awarding Body - Health Research Board Ireland, Name of Scheme - Panel Membership
Year(s) Of Engagement Activity 2011
 
Description Membership Workgroup and Website Workgroup 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Primary Audience Participants in your research or patient groups
Results and Impact Invited Membership . Awarding Body - European Society for Photobiology, Name of Scheme - Committee membership
Year(s) Of Engagement Activity 2012
 
Description Science Foundation Ireland 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Primary Audience Participants in your research or patient groups
Results and Impact Panel Membership . Awarding Body - Science Foundation Ireland, Name of Scheme - Panel Membership
Year(s) Of Engagement Activity 2012