Chromatin disruption and the regulation of V(D)J recombination

Lead Research Organisation: University of Leeds
Department Name: Inst of Integrative & Comparative Biolog

Abstract

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Publications


10 25 50
 
Description BBSRC PhD Studentship
Amount £55,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 11/2005 
End 10/2008
 
Description MRC Project Grant
Amount £395,000 (GBP)
Funding ID G0801101 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 02/2009 
End 06/2012
 
Description YCR Equipment grants
Amount £40,000 (GBP)
Funding ID L443 
Organisation Yorkshire Cancer Research 
Sector Charity/Non Profit
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 01/2009 
End 12/2013
 
Description YCR Project Grant
Amount £144,000 (GBP)
Funding ID L338 
Organisation Yorkshire Cancer Research 
Sector Charity/Non Profit
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 09/2009 
End 03/2014
 
Title Improved method of hypersensitive site mapping 
Description An improved method for analysing active regions in chromatin (hypersensitive sites) was developed and published. Previously, the effects of endogenous nucleases had not been adequately considered; the improved method helps to decrease their impact on hypersensitivity measurements. 
Type Of Material Technology assay or reagent 
Year Produced 2007 
Provided To Others? Yes  
Impact This methodology was made available via its publication. We routinely use this improved technique although it is unclear how many other groups have adopted this modification. 
 
Title Transgenic mouse lines 
Description Transgenic mouse lines were generated where enhancers of V(D)J recombination were mutated to analyse the mechanism by which the enhancer activates recombination. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2006 
Provided To Others? Yes  
Impact The animals were made available to another research group in 2006. A manuscript published in 2013 resulting from the use of these animals. 
 
Title Transgenic mouse lines - increased expression of transcription factor 
Description Transgenic mice that over-express the transcription factors PU.1 or IRF-4/PIP specifically at the pro-B cell stage of development have been generated. The IRF-4/PIP mice induce full activation of immunoglobulin lambda light chain recombination 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact A manuscript describing this work was published in EMBO J and we will make these reagents available to other researchers who request them. 
 
Description Analysis of the regulation of a recombination enhancer 
Organisation Medical Research Council (MRC)
Department MRC Clinical Sciences Centre
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Public 
PI Contribution We made the observations about the role of a transcription factor in regulating the enhancer function and analysed the transgenic mice. Most of the experiments were performed in my lab but with some input and advice from our collaborators in London.
Collaborator Contribution Provided expertise in experiments and performed one key experiment that contributed to the manuscript that is currently under revision
Impact Manuscript being revised for re-submission
Start Year 2006
 
Description Effect of histone acetylation on chromatin remodelling complexes 
Organisation Ludwig Maximilian University of Munich (LMU Munich)
Department University Clinic of Munich
Country Germany, Federal Republic of 
Sector Academic/University 
PI Contribution We made the initial observation and collaborated with two research groups who had reagents/technical expertise to bring this project to publication
Collaborator Contribution Provided technical expertise to bring research finding to publicationProvided chromatin remodelling complexes to bring research finding to publication
Impact This resulted in the publication by Nightingale et al in 2007
Start Year 2006
 
Description Effect of histone acetylation on chromatin remodelling complexes 
Organisation University of Birmingham
Department College of Life and Environmental Sciences
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution We made the initial observation and collaborated with two research groups who had reagents/technical expertise to bring this project to publication
Collaborator Contribution Provided technical expertise to bring research finding to publicationProvided chromatin remodelling complexes to bring research finding to publication
Impact This resulted in the publication by Nightingale et al in 2007
Start Year 2006
 
Description Chromatin Gordon conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A poster was presented at the major International chromatin meeting. This is the most heavily over-subscribed of all the Gordon conferences and most of the poster presenters were PIs. My poster was judged top by a panel of leading International researchers.

Disseminated research findings to researchers in the field
Year(s) Of Engagement Activity 2008
 
Description ESF signalling to chromatin meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A 30 minute talk was given to an International audience that included major researchers in the chromatin field

Disseminated research findings to researchers in the field
Year(s) Of Engagement Activity 2006
 
Description Tenovus Symposium, Scotland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A 30 minute talk was given to an audience that was primarily from the UK but with some from the rest of Europe and beyond.

Disseminated research findings to researchers in the field
Year(s) Of Engagement Activity 2006
 
Description University open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact The work was presented via a poster to parents and students during Univeristy open day

no direct impact known
Year(s) Of Engagement Activity 2007