Development and Use of a Zebrafish Screening Model at Sheffield University

Lead Research Organisation: University of Sheffield

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications


10 25 50
Lipscomb L (2016) Dasatinib as a treatment for Duchenne muscular dystrophy. in Human molecular genetics
Novodvorsky P (2013) Zebrafish-based small molecule screens for novel cardiovascular drugs. in Drug discovery today. Technologies
 
Description BBSRC Excellence with Impact Competition
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
 
Description A* Research Attachment Programme, Singapore, Studentship (VC/PB)
Amount £16,680 (GBP)
Organisation Agency for Science, Technology and Research (A*STAR) 
Sector Public
Country Singapore, Republic of
Start 10/2010 
End 09/2014
 
Description A* Research Partnership Studentship, Singapore (FvE/DG)
Amount £50,000 (GBP)
Organisation Agency for Science, Technology and Research (A*STAR) 
Sector Public
Country Singapore, Republic of
Start 10/2009 
End 09/2013
 
Description Action on Hearing Loss (TW)
Amount £4,825 (GBP)
Organisation The Royal National Institute For Deaf People 
Department Action on Hearing Loss
Sector Charity/Non Profit
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 01/2015 
End 12/2015
 
Description BBSRC Responsive mode Project Grant (TW)
Amount £502,086 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 11/2011 
End 10/2014
 
Description Departmental Studentship (TW)
Amount £58,000 (GBP)
Organisation University of Sheffield 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 10/2014 
End 09/2017
 
Description Marie Curie Intra European Fellowship (TW)
Amount £181,295 (GBP)
Organisation Marie Curie Actions 
Sector Academic/University
Country Global
Start  
 
Description PhD Studentship (VC/PM)/CONACYT (Mexico)
Amount £85,700 (GBP)
Organisation Government of Mexico 
Department National Council on Science and Technology (CONACYT)
Sector Public
Country Mexico, United Mexican States
Start 10/2010 
End 09/2013
 
Description University of Sheffield KT Rapid Response Fund (VC)
Amount £9,519 (GBP)
Organisation University of Sheffield 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 01/2011 
End 12/2011
 
Description University of Sheffield Knowledge Transfer Fund [Karus] (VC)
Amount £9,446 (GBP)
Organisation University of Sheffield 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 01/2010 
End 12/2010
 
Description University of Sheffield POC Fund [Chen] (HR)
Amount £10,000 (GBP)
Organisation University of Sheffield 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 01/2011 
End 12/2011
 
Title A data repository for high throughput screening data (MM) 
Description The system developed is an end-to-end collection, processing and presentation system to support the other sections of this grant. The Data Organisation System (DOS) accepts raw images as produced by optical scanning equipment (primarily microscopes purchased through this award) as input and using an agreed code embedded into the filename or external metadata file. This award inspired the inception of a high performance data storage system in a collaborate effort between researchers from the MRC- CDBG, the Welcome Trust funded RNAi Facility and the Light Microscope Facility. The award used this hardware to implement an advanced file archiving / indexing system based on file fingerprinting such that only links to files are stored in the database and the files are stored on disk. Should the original files move then relinking them correctly in the database is a trivial matter. In the prototype system researchers can view loaded images and assess the images as to whether the result is considered a 'Hit' or whether the compound was considered toxic. The structure of the database has been designed such that all results are attached to a central unique combination of Screen-Plate-compound for library-Well. This allows the database to record more complex quantitative parameters (eg. Fluorescence intensities, absence of fish features, links to external analyses). Details of the compound libraries used for screening are loaded into the database with annotation pertinent to it stored for easy retrieval. A guiding principle of the database is that all the data either loaded in or collected by the interfaces (WWW, SQL access) is stored in flat files as far as is possible and that the entire database can be rebuilt from this using computer code. This allows ease of data integration with other external analysis packages and data entry using familiar spreadsheet interfaces. Template Spreadsheets have been constructed for this purpose that include rudimentary data validation. Further different screens can then be linked together to form 'Investigations' such as primary screen, confirmation of Hits in a smaller replicate screen, a follow-up secondary screen and similar alternative results found in literature searches. Software has been developed based on practical experience gained in the laboratory to identify problems in the library annotation (e.g. multiple compounds listed in the same well, duplicate compounds present, consistency checking of library rearrangements) and present these for further investigation / correction. The chemical structures of compounds in the library are being compared to both each other in the same library, to those in other libraries and publicly databases to identify similar compounds, allow decisions about the overlap in libraries and to improve annotation respectively. The analysis continues but there are clearly patterns within the comparison even when simple atoms connectivity is considered using Open Source Cheminformatics packages (OpenBabel) implementing classical distance measures. Through a collaboration at Erasmus MC (Rotterdam, The Netherlands) an optimally ordered heatmap using specialist software they have access to group compounds together into meaningful clusters has been prepared in preliminary form. This is useful for giving insight to non-domain specialists as to the relationships between compounds contained in libraries. 
Type Of Material Biological samples 
Year Produced 2009 
Provided To Others? Yes  
Impact Support of scientific screens internally. 
 
Title A model of the hypoxia signaling response (FvE) 
Description An assay has been developed using transgenic zebrafish embryos that express GFP as a readout of the hypoxic signaling response. This model and the assay have been exploited to identify novel small molecules that show altered levels of GFP and may therefore have the potential to modulate the hypoxic response therapeutically. The method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Provided To Others? No  
Impact FvE has developed collaborations with companies and other academics. The work has been presented at national and international meetings. Over 2000 small molecules have been screened. 
 
Title A screen for in vivo modifiers of S. Aureus infection (SR) 
Description An assay has been developed using zebrafish embryos to monitor the effects of small molecule libraries on bacterial growth and infection in vivo, using S. aureus. This model and the assay have been exploited to identify novel small molecules that improve infection outcome in vivo and may therefore be new therapeutics for S. aureus infectious disease. This method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Provided To Others? No  
Impact SR has developed collaborations with companies and other academics. The work has been presented at national and international meetings. Over 600 small molecules have been screened.The data have supported successful grant applications. 
 
Title A screen for inflammatory response (SR) 
Description An assay has been developed using transgenic zebrafish embryos, with GFP labelled neutrophils, to monitor the effects of small molecule libraries on the resolution of neutrophilic inflammation after tissue injury, in vivo. This model and the assay have been exploited to identify novel small molecules that reduce the numbers of neutrophils at the site of injury and may therefore be new therapeutics for inflammatory disease. The method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Provided To Others? No  
Impact The method has been published, SR has developed collaborations with companies and other academics. The work has been presented at national and international meetings. Over 5000 small molecules have been screened. A patent application covering this technology is in progress. The data have supported successful grant applications. 
 
Title A screen for modifiers of myelination (JW) 
Description An assay has been developed using zebrafish embryos to identify molecules that modify oligodendrocyte specification in vivo. Embryos were exposed to a drug library from 32 hpf to 52 hpf and then processed for in situ hybridisation staining of olig2 expression to assay for specification of oligodendrocyte precursor cells. This method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Provided To Others? No  
Impact Over 2000 small molecules have been screened. 
 
Title Cryptococcus infection assay (SJ) 
Description The development of a new assay procedure for screening the effects of small molecules on Cryptococcus infection. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact NA 
 
Title Establishment of a drug screening facility (MP) 
Description The drug screening facility is now able to perform all stages of the drug screening pipeline. We have assembled equipment for compound handling and embryo production through to multiple assay designs and hit detection, using a combination of automated and manual methods. In particular we have collaborated with Ash Biotech to increase throughput of embryos into assay plates and hit detection of fluorescent-based assays. We have increased embryo production using a miniMEPS tank (aquatichabitats), increased throughput for the in situ hybridization screen with an in situ hybridization machine (Intavis) and increased hit detection with a customized microscope with a motorized stage from Nikon that is specifically for multiwell plates and can be used for brightfield and fluorescent images. We can screen for behavioural changes using the Zebralab system from Viewpoint 
Type Of Material Improvements to research infrastructure 
Year Produced 2011 
Provided To Others? Yes  
Impact Seventeen assays have been completed using the facility and 10 others are in the pipeline. 
 
Title GPCR agonist drug screen (TW) 
Description An assay has been developed using zebrafish embryos to monitor the effects of small molecules on semicircular canal formation, in vivo. Fish carrying mutations in a GPCR gene develop swollen ears as the semicircular canals fail to form correctly. This assay screens homozygous mutant embryos by in situ hybridization with a marker gene that is normally down-regulated in the ear during semicircular canal development, but remains up-regulated in mutant fish. Molecules that cause down-regulation of the marker gene and rescue the semicircular canal defect in mutant fish can be identified. This method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact 2000 small molecules have been screened; the data have supported a successful project grant application to the BBSRC. 
 
Title GPCR antagonist drug screen (TW) 
Description An assay has been developed using wild type zebrafish embryos to monitor the effects of small molecules on semicircular canal formation, in vivo. Fish carrying mutations in a GPCR gene develop swollen ears as the semicircular canals fail to form correctly. Expression of a marker gene is normally down-regulated in the ear during semicircular canal development, but remains up-regulated in mutant fish. We have screened wild type embryos with a small molecule library and used in situ hybridisation of the marker gene to identify molecules that phenocopy the mutation. These compounds will be tested for an antagonistic role in the relevant signalling pathway. This method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact 2000 small molecules have been screened; the data have supported a successful project grant application to the BBSRC 
 
Title NBT-GCaMP-3 transgenic zebrafish line and plasmid construct (VC) 
Description Transgenic line of zebrafish and plasmid used to generate line. Transgene provides live imaging information on neural activity within the developing CNS. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Year Produced 2012 
Provided To Others? Yes  
Impact None yet; included as preliminary data in grant applications. 
 
Title Quantification of muscle damage in a zebrafish muscular dystrophy model (SW) 
Description A method of analyzing the intensity of GFP fluorescence across the length of the zebrafish somites has been used to distinguish different patterns between wild type zebrafish and zebrafish carrying a mutation in the DMD gene. This method will be used to screen libraries of small molecules for rescue of the mutant phenotype which may lead to the identification of novel drugs for muscular dystrophy. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Provided To Others? No  
Impact This work has been published in PLoS Currents: Muscular Dystrophy. Lipscomb, L., Parkin, C.A., Juusola, M. & Winder, S.J.* (2011) 
 
Title The development of a new assay protocol for screening the effects of small molecules on zebrafish embryos by in situ hybridization (SB) 
Description This protocol uses mesh bottomed plates (Millipore) throughout; from the addition of the drug, through the in situ hybridization machine (Intavis) and the image capture on a customized Nikon microscope. It has a number of advantages over existing methods including increased throughput, reduction in transferring of embryos to reduce the damage to the embryos and sample mixing errors, and high quality automated image capture for hit detection. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have screened 8,000 compounds using this method. 
 
Title Toxicity of anti-prion compounds (BC/HR) 
Description An assay has been developed using zebrafish embryos to monitor the effects of a small molecule library of anti- prion compounds on zebrafish embryo survival, in vivo. This method has been modified to a 96 well format to increase the throughput of screening. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Part of this work has been published (PMID:21726921). 
URL http://europepmc.org/abstract/MED/21726921
 
Title Zebrafish epilepsy model (VC) 
Description A novel model of epileptic seizure induction in zebrafish and a high throughput in situ hybridisation-based assay for seizure-resolving compounds have been developed. This model and the assay have been exploited to identify novel small molecules with anti-convulsant activity and may therefore have potential as anti-epileptic drugs. 
Type Of Material Model of mechanisms or symptoms - non-mammalian in vivo 
Provided To Others? No  
Impact Selected Oral Presentation at EUFishBiomed COST-ACTION Workshop on Zebrafish Neurophysiology an Behaviour. Queen Mary University of London 2010. HDAC inhibitor screen in zebrafish (Jan-June 2010). A collaboration with Karus Therapeutics funded by University of Sheffield Knowledge Transfer Project Fund. 
 
Description Customized microscope (SB) 
Organisation Nikon
Country Global 
Sector Private 
PI Contribution Collaborated to produce a customized microscope for automated imaging of bright field and fluorescent images in multiwall plates
Collaborator Contribution Collaborated to produce a customized microscope for automated imaging of bright field and fluorescent images in multiwall plates.
Impact NA
Start Year 2009
 
Description Development of new equipment to increase throughput of drug screening methods in zebrafish (SB) 
Organisation ASH Biotech
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Private 
PI Contribution Collaboration to produce new equipment for increasing throughput of drug screening methods in zebrafish. Over the past year we have contined to be involved in the product specification and design and testing of the equipment.
Collaborator Contribution Increasing throughput of drug screening methods in zebrafish. The collaboration resulted in two new pieces of equipment being developed. The Phenosight imaging system has been modified over the past year and the software is much improved. The embryo dispenser has also improved but is still in the beta version.
Impact Beta versions of two pieces of equipment are being evaluated. The Phenosight fluorescent plate reader that can take high speed images of transgenic zebrafish in multiwell plates and record the level of GFP in each embryo is now ready for marketing by ASH. This equipment is used extensively in the screening unit. An embryo dispenser that can accurately aliquot embryos into 96 well plates is being optimized. The beta version works well for 48 hpf embryos.
Start Year 2009
 
Description Epigenesys (SB) 
Organisation University of Sheffield
Department epiGenesys Ltd
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Private 
PI Contribution We have been involved in the specification and design of the database.
Collaborator Contribution EpiGenesys have proposed to expand the current capability of the screening unit database.
Impact The "Screener" database proposed is being evaluated and is ready to be further developed.
Start Year 2011
 
Description HDAC inhibitor screen in Zebrafish [Karus Therapeutics] (VC) 
Organisation Karus Therapeutics
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Private 
PI Contribution Characterisation of novel HDAC inhibitors as potential anti-epileptic drugs using molecularly focused screen in zebrafish.
Collaborator Contribution Characterisation of novel HDAC inhibitors as potential anti-epileptic drugs using molecularly focused screen in zebrafish.
Impact NA
Start Year 2009
 
Description Seizure Liability Screen [AstraZeneca] VC 
Organisation AstraZeneca
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Private 
PI Contribution Carried out all of the experimental work to assay the compounds provided by AstraZeneca.
Collaborator Contribution AstraZeneca provided a set of known seizure-inducing compounds of varying potencies, which were evaluated in our novel assay for seizure liability. This work was able to identify seizure liability in some compounds that were not detected by AstraZeneca's existing assays. We concluded that for some compounds our assays are more sensitive than the one currently used by AstraZeneca
Impact A grant application is being developed.
Start Year 2011
 
Description UoS Chen (HR) 
Organisation Faculty of Science
Department Department of Chemistry
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Provided expertise in zebrafish research.
Collaborator Contribution Expertise.
Impact PMID: 21726921.
Start Year 2010
 
Title Compounds with anticonvulsant activity in zebrafish (VC) 
Description A re-purposing screen of a library of known bioactive compounds was screened for anticonvulsant activity in zebrafish embryos. Several compounds were identified that had not previously been shown to exhibit anti-convulsant activity. 
IP Reference  
Protection Protection not required
Year Protection Granted 2012
Licensed No
Impact Increased interest in the epilepsy research community in using the zebrafish as a model organism for epilepsy studies.
 
Description A Question of Taste; Hands on DNA post 16 workshop (SB/CP) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Type Of Presentation Workshop Facilitator
Geographic Reach Local
Primary Audience Schools
Results and Impact A day long laboratory practical course in molecular biology and genetics for sixth form students.
36 Biology students from 2 schools attended the day and met scientists and learned about how we use molecular biology to understand genetics.
The workshop is offered with Science Brainwaves in collaboration with the University of Sheffield and the ASDC (The UK Association for Science and Discovery Centres), and supported by the Wellcome Trust. During this one-day workshop, students are able to explore the evolution of an unusual human trait where some people can taste a bitter chemical, whilst others cannot. Students use molecular biology techniques (DNA extraction, Polymerase Chain Reaction, restriction digestion and gel electrophoresis) to test their own DNA to compare their ability to taste a bitter chemical to the allele (version) of the responsible taste receptor gene.
The workshop is run on average once a month to a class of 12-24 students. There is a lot of opportunity for dialogue about the science, current research and the ethics of genetic testing. The students also see the University's working labs, as the workshop is run in our undergraduate teaching laboratories.

This was a second visit for the schools and there are visits from many local schools planned throughout the year.
The feedback forms show that around 70% of students are more likely to consider carrying on with science at degree level after attending the class. They also have a better understanding of what a scientist does. Most teachers have asked to return with the following year's group of students.
Year(s) Of Engagement Activity 2011,2012
 
Description Biology Workshop for sixth form students (SB) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Type Of Presentation Workshop Facilitator
Geographic Reach Local
Primary Audience Schools
Results and Impact 30 sixth form Biology students visited our department for a biology workshop, to learn about biomedical research in the university.
This workshop focused on how researchers use zebrafish in biomedical research. There was a presentation, a visit to the aquarium and observation of zebrafish embryos using microscopy. They also met informally with PhD students.

This is the second year we have run the workshop and we will run it again next year. We had very positive feedback from the students and teachers who all seemed to get a lot out of the day.
Year(s) Of Engagement Activity 2012
 
Description Discovery Night Festival of Science and Engineering (FvE/TW) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Enthused and educated public in the area of our research.

Future participation planned.
Year(s) Of Engagement Activity 2014
 
Description Disease, Development and Disco Fish - Pecha Kucha Night (CP) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation Keynote/Invited Speaker
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact 250 people attended a short talk, presented in a lively and engaging way explaining the use of zebrafish in medical research, under the heading Fish For Science.

Received excellent feedback from the audience and talked late into the night with many people about the research. Lots of interesting conversations on twitter followed from the talk.
Invited to give further talks at 'creative' nights in the city, including International Women's Day and TedX.
Year(s) Of Engagement Activity 2011
 
Description Environmental Robots activity (SB) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Enthused and educated school children in the area of our research.

Similar future activities planned.
Year(s) Of Engagement Activity 2014
 
Description Festival of health, from head to toe (SR) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Enthused and educated public in the area of our research.

Future events planned.
Year(s) Of Engagement Activity 2014
 
Description Fish for Science website (CP/SB) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Production of the FishforScience website.

Awarded first Wednesday award by Understanding Animal Research. They quoted "This great website explains science very clearly and includes excellent teaching resources."
Year(s) Of Engagement Activity 2009,2010,2011,2012
 
Description Innovations in Healthcare (VC) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Health professionals
Results and Impact Enthused and educated public in the area of our research.

Tours of facilities arranged.
Year(s) Of Engagement Activity 2014
 
Description King Edwards Sixth Form Biology Workshop (SB) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Talk and activities explaining why we use fish for research, involving looking at fish embryos down the microscope for approximately 80 pupils.

2013 - 24 biology students attended a workshop on how researchers use zebrafish in biomedical research, as part of a day of talks and activities to inspire then to work hard at their A levels.
They listened to a presentation, visited the aquarium and observed zebrafish embryos using microscopy.

Pupils asked lots of questions.
Feedback from a teacher - "The feedback from the students that attended your workshop was excellent, with students highlighting that the session was fun, informative and engaging - especially the research involving the zebra fish. It is clear that the students had a great insight into how research takes place and that they were very interested in looking at and studying the different stages of a cells development."
Year(s) Of Engagement Activity 2011,2012,2013
 
Description Magna Science Centre 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Young Chambers Science and Technology week at Magna Science Adventure Centre.
"Fishing for clues: why medical researchers are glowing with excitement". Members of the Centre for Developmental and Biomedical Genetics presented their research to approximately 200 GCSE students and members of the public. We demonstrated our research using live zebrafish embryos (fluorescence microscopy), together with video and written presentations.

Enthused students with out research.
Year(s) Of Engagement Activity 2010
 
Description Primary School Visit, Sheffield (SB) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Talk explaining why we use fish for research.

Children asked lots of questions.
Year(s) Of Engagement Activity 2011
 
Description Primary School visits - Sheffield (SB/CP) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Three primary school visits - Sheffield.
Talk and activities explaining why we use fish for research, involving looking at fish embryos down the microscope for approximately 150 pupils.



We have been asked to come back to two schools to give the same presentation next year and also to work with the GCSE students.
Year(s) Of Engagement Activity 2010
 
Description Public Engagement Competition (CP) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Yorkshire and North East Hub Public Engagement Competition, Newcastle-upon-Tyne.
Gave a talk "Taking lessons from zebrafish to heart".

NA
Year(s) Of Engagement Activity 2010
 
Description Researcher Night 2016 (J-PA) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Researchers night, a Festival of the Mind event, hosting talks, demonstrations and exhibitions based around University of Sheffield research. The event was open to all and is free to attend.
Year(s) Of Engagement Activity 2016
 
Description School visit - King Edward VII, Sheffield 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Presentation to year 11s about current research in Sheffield using non mammalian models, particularly Drosophila, to research human diseases.

Outcomes not yet determined. One student wrote on feedback form that s/he wants to be a PhD student.
Year(s) Of Engagement Activity 2009
 
Description UCAS Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Enthused and educated public in the area of our research.

Increased interest from students wishing to study science.
Year(s) Of Engagement Activity 2013,2014
 
Description Using zebrafish for biomedical research (SB) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Increased interest in research.

Further visits planned in future years.
Year(s) Of Engagement Activity 2014